Ccr8 ^−/−mice are impaired in N. brasiliensis defense due to defective type 2 immune responses. (A–L) Mice of indicated genotypes were infected with 500 L3 N. brasiliensis (N.b.) larvae. (A) Serum concentrations of CCL1 in WT mice were measured in response to N. brasiliensis infection at 9 dpi. (B and C) Expression of Ccl1 and Ccr8 was determined in naive (control) and N. brasiliensis–infected WT, Ccr8^−/−, and Tie2^creRora^fl/sg lungs by qPCR. One-way ANOVA was applied. (D) Parasite eggs in stool samples of WT and Ccr8^−/− mice were counted 6 dpi to 11 dpi. (E) Adult worm counts in small intestinal tissues were determined 9 dpi. (F–J) ILC2 (GATA3⁺Thy1⁺Lin⁻), eosinophil (CD11b⁺SiglecF⁺SSC^hi), T reg cell (CD4⁺FoxP3⁺), neutrophil (CD11b⁺Ly6G⁺), and Th2 cell (GATA3⁺Thy1⁺CD4⁺Lin⁺) numbers per lung were determined by flow cytometry 9 dpi. (K and L) Expression of the type 2–related effector cytokines Il5, Il13, and Il9 (K) as well as the mucins Muc5ac and Retnlb (L) were determined in lung and small intestinal (SI) tissues by qPCR 9 dpi. All graphs show pooled data of two to four representative experiments with at least four mice in each experimental group. Each dot represents one animal. Data represent mean ± SEM. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; Mann–Whitney U tests or, if indicated, one-way ANOVA.