CCR6 is required for efficient B cell entry into the LN cortex. (A) Representative LSFM 3D-rendered images of LNs removed from AID.Cre.tdTomato or AID.Cre.tdTomato CCR6^−/− mice 12 d after immunization with NP-OVA. Scale bar, 200 μm. (B–E) Analysis of the number of individual cells per LN (B), GC volume (C), number of non-GC individual cells normalized to the total GC volume (D), and proximity index (E). Representative images and data for A–E were pooled from three to five independent experiments with a total of 9–18 LNs from five to nine mice per group. *, P < 0.05; ***, P < 0.0001; two-tailed Student's t test.