Figure 1. P2Y1R expression in AD and... | Rockefeller University Press

Figure 1.

$Figure 1. P2Y1R expression in AD and APPPS1 mice. (A) P2Y1R expression in cortical astrocytes (anti-GFAP; arrows) in human AD. Right: P2Y1R expression occurred in the majority of GFAP-positive astrocytes in cortex (CX) and hippocampus (HC; n = 211 cortical and 106 hippocampal astrocytes from four AD patient samples; mean ± SEM). (B) Reactive astrocytes (GFAP) around Aβ plaques (stained with IC16 antibody; arrow) in the cortex in human AD express P2Y1R (arrowheads). (C) In APPPS1 mice, P2Y1R are expressed by reactive astrocytes (arrows) around plaques (labeled with methoxy-X04) as well as neurons (arrowheads). Bars, 50 µm. (D) The majority of P2Y1R-positive cells were astrocytes, whereas neurons accounted for a smaller fraction (data are from n = 4 APPPS1 mice; age, 6 mo). (E and F) APPPS1 showed an age-dependent increase of P2Y1R concentration (Spearman correlation, ρ = 0.73) that correlated with progressive reactive astrogliosis (GFAP; ρ = 0.63). No increase was seen in WT littermates (ρ = 0.22; n = 9 APPPS1 vs. n = 11 WT mice).$

P2Y1R expression in AD and APPPS1 mice. (A) P2Y1R expression in cortical astrocytes (anti-GFAP; arrows) in human AD. Right: P2Y1R expression occurred in the majority of GFAP-positive astrocytes in cortex (CX) and hippocampus (HC; n = 211 cortical and 106 hippocampal astrocytes from four AD patient samples; mean ± SEM). (B) Reactive astrocytes (GFAP) around Aβ plaques (stained with IC16 antibody; arrow) in the cortex in human AD express P2Y1R (arrowheads). (C) In APPPS1 mice, P2Y1R are expressed by reactive astrocytes (arrows) around plaques (labeled with methoxy-X04) as well as neurons (arrowheads). Bars, 50 µm. (D) The majority of P2Y1R-positive cells were astrocytes, whereas neurons accounted for a smaller fraction (data are from n = 4 APPPS1 mice; age, 6 mo). (E and F) APPPS1 showed an age-dependent increase of P2Y1R concentration (Spearman correlation, ρ = 0.73) that correlated with progressive reactive astrogliosis (GFAP; ρ = 0.63). No increase was seen in WT littermates (ρ = 0.22; n = 9 APPPS1 vs. n = 11 WT mice).