Tγδ17 cells are long lived and self-renewing. (A) Inducible γδ T cell fate-mapping model. RFP expression in γδ T cells was induced by oral administration of tamoxifen (left). Representative gating for RFP⁺ peripheral lymph node γδ T cells 7 wk after tamoxifen administration (right). (B and C) Analysis of RFP⁺ cells from indicated tissues and time points after RFP induction. One dot equals one mouse, pooled data from three independent experiments with each three to six littermate mice per time point, mean. (B) Frequencies of RFP⁺ γδ T cells. (C) Frequencies of RFP⁺ cells among IFN-γ (left) or IL-17 (right) phenotype γδ T cell subsets, defined as CD3⁺Tcrβ^–γδTCR⁺CD44^hiCD27^– and CD3⁺Tcrβ^–γδTCR⁺CD44^–CD27⁺, respectively. (D) Mean frequency of RFP⁺ cells among skin-derived γδTCR^hi DETCs and γδTCR^lo dermal γδ T cells. One dot equals one mouse, pooled data from two experiments with each three to five littermate mice per time point, mean.