Depletion of Tγδ17 cells does not change their repertoire. (A and B) Flow cytometric analysis of indicated cell populations 1 d (d1), 2 wk (2w), and 7 wk (7w) after depletion of γδ T cells in Tcrd-GDL littermate mice. Shown are pooled data from three independent experiments with each n = 2 - 5 mice per group, Student’s t test. (A) Bar graph shows frequencies of γδ T cells (Tcrβ^–GFP⁺) among T cells (A.dead^–CD3⁺) in peripheral lymph nodes, mean ± SD. (B) Scatter plots show frequencies of indicated γδ T cell populations among all γδ T cells in peripheral lymph nodes, one dot represents one mouse, mean. (C) T cell receptor repertoire analysis of γδ T cell nondepleted (ctrl., left) and depleted Tcrd-GDL mice 8–9 wk after depletion (right). Sorted CD44^hiLy6C^–Vγ4⁺ peripheral lymph node cells were pooled from 7 - 8 mice each and facilitated to Illumina NGS. Amplicons were generated by multiplex PCR. Proportions of Vγ4 (top) and Vδ5 (bottom) top 20 and top 5 amino acid (AA) clonotypes among complete Vγ4 and Vδ5 repertoires. Amino acid sequences of the top 5 clonotypes. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.