Multi-organ infiltration of neutrophils in the steady-state. (A) Micrographs of tissues illustrating the presence of neutrophils (green; shown by yellow arrowheads) in Lyz2^GFP reporter mice. These GFP^HI cells also stained for Ly6G (see Fig. S1 B). Insets show details of neutrophils within each tissue. Bars: 100 µm (main images); 10 µm (inset images). (B) Neutrophils infiltrate multiple organs from the circulation, as observed in parabiotic pairs (left scheme). Representative images of Lyz2^GFP neutrophils present in tissues of the nonfluorescent WT partner mouse. Bars: 50 µm (main images); 10 µm (inset images). (C) Quantification of partner-derived neutrophils per organ (except for blood, which represents 1 ml; for BM, one femur; for skin and muscle, 100 mg); n = 8–18 from three independent experiments. (D) Neutrophil chimerism in tissues of the nonfluorescent partner from WT with Lyz2^GFP parabiotic pairs; n = 20–22 from two independent experiments. Note that chimerism in tissues equilibrates with that in blood, except for the BM. Error bars show mean ± SEM values.