Anti-VEGF targets Res-TAM and MoD-TAM accumulation. (A) Bone marrow monocytes from MacBlue mice were adoptively transferred in tumor-bearing WT mice treated or not with anti-VEGF (upper panel). Representative overlay dot plots show the phenotype of recovered MoD cells from tumor-bearing mice treated (green) or not with anti-VEGF (black) 24 h after transfer (lower panels). (B) Graph shows the relative proportion of recovered cells in each mouse pooled from two independent experiments (black bars indicate means; two-way ANOVA with Bonferroni multiple comparisons test was performed). (C) Graph shows the proportion of Ly6C^high-Mo infiltration evaluated by blood/tissue partitioning in each mouse pooled from two independent experiments (black bars indicate means; Student’s t test was performed). (D) Representative histogram plot of FTL1 expression by ECFP⁺ and ECFP⁺-TAMs, mean percentage ± SD of FLT1⁺ cell in each subset out of six mice from two independent experiments are indicated. (E) Left panel shows the number of TAMs/milligram in indicated conditions. Right panel shows the relative proportion of TAMs in mice treated with anti-VEGF or isotype. Black bars indicate means of two independent experiments, Student’s t test was performed. For all panels: *, P < 0.05; ****, P < 0.0001; ns, not significant. See also Fig. S5.