Anti-VEGF combination with CP reduces TAM recovery and enhances chemotherapy efficacy. (A) Tumor-bearing mice were treated with CP 10 d after tumor inoculation and treated or not every 2 d with anti-VEGF. (B) Representative tSNE dimension 1 and 2 plots show the impact of the different therapies on the myeloid signature at day 15. Cell subsets are color grouped, and dashed black gates delineate blood/tissue partitioning. (C) Blood/tissue partitioning monitoring of lung monocytes and macrophages during tumor growth at days 15 and 20 after CP or CP + anti-VEGF. (Bars represent mean of the total number of cell ± SEM. For all panels n = 6 mice per time point out of two independent experiments; two-way ANOVA with Bonferoni multiple comparisons test was performed.) (D) Survival curve shows the efficacy of combined therapy started from day 20 (n = 7 mice per group, data are representative of two experiments; log-rank [Mantel-Cox] test was performed to compare each survival curve with the one of CP + anti-VEGF). Corresponding lung weights are reported (one-way ANOVA with Bonferroni multiple comparisons test was performed). For all panels: *, P < 0.05; **, P < 0.01; ***, P < 0.001.