Lung IMs are of embryonic origin and accumulate within tumors. (A) Dot plots show tdTomato⁺ myeloid cell subsets in Csf1r^MeriCreMer; Rosa^LSL-tdTomato after OH-TAM pulse at E8.5 in adult tumor-free mice and 15 d after TC-1 inoculation (left panels). Mean percentage ± SD of tdTomato⁺ cells among each subset is indicated. Right panel shows the numbers per milligram of tissue of the tdTomato⁺ AMs and Ly6C^low/- CD64⁺ Mac. Bars represent mean of four mice per group out of two independent experiments. Two-way ANOVA with Bonferroni multiple comparisons test was performed. (B) Dot plots show YFP⁺ macrophage subsets in Tnfrsf11a^Cre; Rosa26^LSL-YFP mice. Mean percentage ± SD of YFP⁺ cells among each subset is indicated (left panels). Right panel shows the absolute number per milligram of YFP⁺ AMs and Ly6C^low/- CD64⁺ Mac. Bars represent mean of four mice per group out of two independent experiments. Two-way ANOVA with Bonferroni multiple comparisons test was performed. For all panels: *, P < 0.05; ***, P < 0.001.