Lung macrophage subsets differentially accumulate during tumor development. (A) Representative tSNE dimension 1 and 2 plots of the lung myeloid compartment evolution after TC-1 cell intravenous inoculation. Upper panel delineates cell blood/tissue partitioning (dashed gates). Color clusters are represented over time (lower panels). (B) Blood/tissue partitioning monitoring of lung monocytes and macrophages during tumor growth. Dots represent mean of the absolute number ± SEM/milligram of tissue (upper panels) or absolute number per whole lung (lower panels). Statistical differences are indicated compared with D0. For all panels, n = 6–8 mice per time point out of three independent experiments, two-way ANOVA with Bonferroni multiple comparisons test was performed. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). See also Figs. S1.