Eosinophils contribute to intravascular thrombosis and hemostasis. (A) Representative microscopy image (n = 5) showing immunofluorescence staining for platelets (CD41; green), eosinophils (CCR3; red), and cellular nuclei (DAPI; blue) in a ferric chloride (FeCl)–induced thrombus of a mouse IVC. White arrowheads indicate CCR3⁺ eosinophils, and dashed lines mark lacunae surrounded by platelet aggregates. Bar, 100 µm. (B) Relative number of eosinophils in IVC thrombi of WT mice compared with peripheral blood counts. n = 5. (C and D) FeCl-induced IVC thrombus of ΔdblGATA1 mice (BALB/c background; n = 4; C), PHIL mice (C57BL/6 background; n = 10; D), and their corresponding WT littermates. Bar, 2 mm. (E) FeCl-induced IVC thrombosis in WT mice treated with anti-SiglecF antibody (n = 4) or isotope control. (F) Measurement of TAT complexes in plasma from dblGATA1 mice (n = 5) after FeCl-induced IVC thrombosis. (G) Representative image of a FeCl-induced thrombosis of the carotid artery in WT or ΔdblGATA1 mice (30 min after FeCl-induced injury) and quantification of the kinetics of thrombus formation and dissolution (right; see also supplementary videos). n = 4. Bars, 200 µm. (H) Bleeding assays (15-mm tail cut) with WT (BALB/c; n = 5) and ΔdblGATA1 mice (n = 9). Bar graphs show relative weight loss, OD_575nm of the lost blood after lysis, and primary bleeding time (time until the first stop of bleeding). Data are representative of at least three independent experiments. Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; Student’s t test.