Steady-state neutrophil populations in the splenic RP. (a) Representative time-lapse images of mobile (white arrowheads) and immobilized (encircled; green arrowheads) neutrophil populations in the splenic RP under basal conditions. Red, neutrophils; blue, vasculature. Bar, 50 µm. Data are representative of n = 5 from three independent experiments. (b) Cellular tracks representation of mobile and immobilized neutrophil populations from representative time-lapse images. (c) Quantification of cell velocity distribution of mobile (dark blue bars) and immobilized (light blue bar) neutrophils in the splenic RP under basal conditions. There were four fields of view (FOV) per animal. n = 5 from three independent experiments. (d) Representative flow cytometry plots (blood and spleen), IVM images (spleen), and quantification of neutrophil origin after parabiosis. SSC, side scatter. Bars, 25 µm. There were four fields of view per animal. n = 4 from three independent experiments. *, P < 0.05. Student’s t test statistical analyses were performed. Data are represented as mean ± SEM.