Pivotal role of Skap2 for neutrophil recruitment during sterile inflammation. (A–E) Neutrophil recruitment of WT and Skap2^−/− mice in response to focal hepatic necrosis. (A) Adhesion of neutrophils to the microvascular endothelium after injury or sham operation. n = 4 mice/group. (B) Adherent neutrophils within the indicated regions around foci of necrosis 4 h after injury. n = 4 mice/group. (C) Representative time-lapse images of neutrophils (green) and area of focal hepatic necrosis (red). (D and E) Serum levels of GOT (D) and GPT (E) 4 h after injury or sham operation. n = 4 mice/group. (F–J) Neutrophil recruitment of WT and Skap2^−/− mice in response to renal IRI. (F) Creatinine serum levels 24 h after IRI or sham operation. n = 4 mice/group. (G) Neutrophil numbers per kidney 24 h after IRI or sham operation. n = 4 mice/group. (H) Representative hematoxylin and eosin staining of kidney outer medulla 24 h after IRI or sham operation. (I) Acute tubular necrosis scores in kidneys 24 h after IRI or sham operation. n = 4 mice/group. (J) Neutrophil counts in blood 24 h after IRI or sham operation. n = 6 mice/group. *, P < 0.01; **, P < 0.001; Student's t test. See also Videos 1 and 2. Data are means ± SEM.