foxp3a mutations and effects on survival. (A) Gene structure of foxp3a with sites and sequences of mutations indicated. (B) qRT-PCR of foxp3a in WKM relative to nonhematopoietic fin tissue. foxp3a(um252) animals show a reduction of foxp3a transcripts. Error bars indicate SEM; n = 3. (C) Kaplan–Meier survival analysis of foxp3a mutants compared with wild-type animals. Log-rank (Mantel–Cox) test, *, P < 0.0001 for pairwise comparisons of foxp3a(um252/um252) (n = 37), foxp3a(um253/um253) (n = 33), and foxp3a(um252/um253) (n = 54) versus wild type (n = 59).