![Figure 3. Expression analyses of foxp3a:EGFP-positive cells. (A) Heat map of T reg signature genes as defined by Sugimoto et al. (2006). Mouse gene names are indicated. Only genes with a normalized read count >10 are shown, as are genes with unambiguous zebrafish-to-mouse orthology, except for Foxp3, for which expression values of foxp3a are represented. (B) Top, GSEA showing that expression of a T reg signature, as defined by Sugimoto et al. (2006), is enriched in zebrafish foxp3a:EGFP cells. Bottom, GSEA showing enrichment of a broader T reg signature, as defined by Hill et al. (2007). (C) Violin plots from single-cell analyses showing that the expression profile of foxp3a:EGFP-positive cells is biased to that of mature (TCR+) cd4-1+cd8a– lymphocytes. Cell populations were assigned from single-cell analyses of sorted lck:EGFP-positive (Lck) and thymic (double-negative [DN], double-positive [DP], CD4, and CD8) cells. By ANOVA, foxp3a (P = 1.61 × 10–22), cd4-1 (P = 1.02 × 10–36), cd8a (P = 1.35 × 10–58), TCRα (P = 2.60 × 10–6), and TCRβ (P = 5.18 × 10–8).](https://cdn.rupress.org/rup/content_public/journal/jem/214/12/10.1084_jem.20162084/6/jem_20162084_fig3.jpeg?Expires=1771101324&Signature=NEo25kNN1WLddXV39QORwhaA6xCy2FCGnF-0XS-WFmfC8ktqV65rq0w5PFPBMG2tCU-jZDModvphckIa9rrjvYWzn5Gc-mSKeVEGI7tEa1-aGXfN4i5aJ6Nw-KoI5fNd6ynqEx59GhDTIvU3ayDZkx7HQqBFE6Zh3Lbzs8f8mzNY-DURoO59jkuiXzZV86VrLWT8tgDEmfWnSmRIExkIMik1ITfxJ3e23CCOaIMJJVwdMOAQNpB8uxWN2zUAQGc3QZgIcygDt8ctiAskXd1C0Eo7tSteDCnvnamfJ5mwktqZgI8MzLOqLwB-ICWe1UQKk2RrUwiEyY-uK5W5tr6Nng__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression analyses of foxp3a:EGFP-positive cells. (A) Heat map of T reg signature genes as defined by Sugimoto et al. (2006). Mouse gene names are indicated. Only genes with a normalized read count >10 are shown, as are genes with unambiguous zebrafish-to-mouse orthology, except for Foxp3, for which expression values of foxp3a are represented. (B) Top, GSEA showing that expression of a T reg signature, as defined by Sugimoto et al. (2006), is enriched in zebrafish foxp3a:EGFP cells. Bottom, GSEA showing enrichment of a broader T reg signature, as defined by Hill et al. (2007). (C) Violin plots from single-cell analyses showing that the expression profile of foxp3a:EGFP-positive cells is biased to that of mature (TCR+) cd4-1+cd8a– lymphocytes. Cell populations were assigned from single-cell analyses of sorted lck:EGFP-positive (Lck) and thymic (double-negative [DN], double-positive [DP], CD4, and CD8) cells. By ANOVA, foxp3a (P = 1.61 × 10–22), cd4-1 (P = 1.02 × 10–36), cd8a (P = 1.35 × 10–58), TCRα (P = 2.60 × 10–6), and TCRβ (P = 5.18 × 10–8).
