Figure 2.
Figure 2. Identification and analysis of foxp3a:EGFP-positive thymocytes. (A) Flow cytometry analysis of thymus from representative AB and Tg(foxp3a:EGFP) animals. (B) qRT-PCR of selected genes in foxp3a:EGFP-positive thymocytes relative to bulk thymocytes. Relative CD4 and CD8 expression is also shown and represents zebrafish cd4-1 and cd8a genes. †, Expression of cd8a was below the limit of detection in foxp3a:EGFP-positive thymocytes. Error bars indicate SEM.; n = 3. (C) Cells from dissected thymus of a Tg(foxp3a:EGFP); Tg(rag2:mCherry) animal showing a double-positive thymocyte (arrowheads). Bars, 10 µm.

Identification and analysis of foxp3a:EGFP-positive thymocytes. (A) Flow cytometry analysis of thymus from representative AB and Tg(foxp3a:EGFP) animals. (B) qRT-PCR of selected genes in foxp3a:EGFP-positive thymocytes relative to bulk thymocytes. Relative CD4 and CD8 expression is also shown and represents zebrafish cd4-1 and cd8a genes. †, Expression of cd8a was below the limit of detection in foxp3a:EGFP-positive thymocytes. Error bars indicate SEM.; n = 3. (C) Cells from dissected thymus of a Tg(foxp3a:EGFP); Tg(rag2:mCherry) animal showing a double-positive thymocyte (arrowheads). Bars, 10 µm.

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