DC-to-MC communication shifts from dynamic scanning to firm and long-lasting contacts in the progression of skin inflammation. (A–D) Sequences of intravital time-lapse series (3D-rendered z-stack) of DC/MC pairs before and after DNFB administration. Bars, 10 µm. Refers to Videos 5, 6, 7, 8, 9, 10, 11, and 12. (E) Computed quantification of DC-to-MC contact duration normalized to observation time (contact ratio) and DC/MC overlap change over time before (n = 50 cell tracks) and after DNFB administration (n = 89 [10 ± 2 h], n = 99 [26 ± 2 h]). Histograms represent the normalized number of cell tracks with a certain contact ratio or overlap change. *, P < 0.05; **, P < 0.01.