DNFB-driven skin inflammation induced changes in MCs shape and dynamics. (A) Intravital multiphoton imaging of MC^RFP reporter mouse ear skin before and 24 h after DNFB administration, MIP. Bars, 50 µm. (B and C) Computed quantification of the MC size (B) and eccentricity (C) in randomly chosen ROI z-stacks of DC^GFP/MC^RFP mice before (n = 102 z-stacks, 8652 cells) and after DNFB (n = 30 z-stacks each, 1,951 and 2,211 cells). Histograms represent the normalized number of MCs with a certain size or eccentricity. (D and E) Dynamic change in MC shape over time of observation in time-lapse series of DC^GFP/MC^RFP mice ear skin before (n = 168 cell tracks) and after DNFB administration (n = 279 and 218 cell tracks). Histograms represent the normalized number of cell tracks with a certain shape change dynamic of MCs. ***, P < 0.001.