Dermal DC paralysis and remobilization in responses to DNFB. (A) Maximum intensity projection DC^GFP/MC^RFP mouse ear skin representing DCs (green), MCs (red), and blood vessels (blue) before and after DNFB administration at one time point of the corresponding 30-min time-lapse series (Video 1). Bars, 30 µm. (B and C) Kinetic overlay of the DC (B) and MC (C) channel at three consecutive time points (1, 15, and 30 min). Motile cells are colored and immobile cells are white. Bars, 30 µm. (D) Determination of observation time frames according to DC motility. For detailed analysis of MC motility in the determined time frames, see Fig. 3.