KCs are essential for capturing MRSA from circulation. (a) Staphylococcal dissemination to different organs 30 min after i.v. infection (n = 6 mice; data were pooled from two independent experiments). (b) SD-IVM image of staphylococcal (MW2-GFP; green) catching by KCs (F4/80; purple) in WT (left) or KC depleted liver (right; Video 2). Bars, 50 µm. (c) Enumeration of staphylococcal catching by KCs in the livers of WT mice or CCL-treated mice. (b and c) n = 4 mice; thin lines, SEM. Data were pooled from two independent experiments. (d) Quantification of staphylococcal catching by KCs in mice infected with various S. aureus strains. Black, CA-MRSA strains (MW2 and USA300); gray, HA-MRSA strains (Col and N315); red, MSSA strains (Newman and SH1000). n = 3 per condition. Data were pooled from three independent experiments. (e) Staphylococcal bacteremia 4 h after i.v. infection with MRSA (MW2) in untreated or KC-depleted mice by CCL treatment. n = 5 per treatment group; **, P < 0.01, Student’s t test. (f) Survival of MRSA (MW2)-infected WT mice or CCL-treated mice. n = 5 mice per treatment group; log-rank test. (g) Staphylococcal CFU at various time points after i.v. infection with MRSA (MW2) in liver and kidney. n = 6–8 mice per time point. Data shown are compiled from three independent experiments.