Figure 5. IFNL4 mRNA are poorly loaded... | Rockefeller University Press

Figure 5.

$Figure 5. IFNL4 mRNA are poorly loaded onto polyribosomes for translation. (A) Whole-cell extracts from mock- or poly(I:C)-treated HepG2 cells were resolved by density sedimentation in 10–50% sucrose gradients. The UV absorbance trace (254 nm) obtained during fractionation is shown with the positions of the 40S, 60S, 80S, and polyribosomes. The bottom panel shows an agarose gel of polysome fractions to check for 28S and 18S ribosomal RNA in the fractions. (B–D) Copy number expression of IFNL3 and IFNL4 isoforms in input (B), monosome (C), and polysome (D) fractions measured by qPCR. (E) Cleavage sites (CS) and frequency of CS usage in the human IFNL4 3′ UTR determined by 3′ RACE. (F) Gene expression of IFNL3 and IFNL4 isoforms in gorilla fibroblasts upon stimulation with poly(I:C). Representative of two to three independent experiments.$

IFNL4 mRNA are poorly loaded onto polyribosomes for translation. (A) Whole-cell extracts from mock- or poly(I:C)-treated HepG2 cells were resolved by density sedimentation in 10–50% sucrose gradients. The UV absorbance trace (254 nm) obtained during fractionation is shown with the positions of the 40S, 60S, 80S, and polyribosomes. The bottom panel shows an agarose gel of polysome fractions to check for 28S and 18S ribosomal RNA in the fractions. (B–D) Copy number expression of IFNL3 and IFNL4 isoforms in input (B), monosome (C), and polysome (D) fractions measured by qPCR. (E) Cleavage sites (CS) and frequency of CS usage in the human IFNL4 3′ UTR determined by 3′ RACE. (F) Gene expression of IFNL3 and IFNL4 isoforms in gorilla fibroblasts upon stimulation with poly(I:C). Representative of two to three independent experiments.