Complement facilitates efferocytosis of PITs in vivo. (A) WT BMMs (CellTracker Green) and Casp-1^−/−Casp-11^−/− BMMs (CellTracker Blue) were co-cultured, imaged, and analyzed as in Fig. 4 C, and treated with 2-ThioUTP. (B, G, and H) Mice (five animals per group) were infected with 1:1 ratio of FliC^ind Amp^R and WT Kan^R S. Typhimurium IP for 17 h before doxycycline, fucoidan, and 2-ThioUTP injection; organs were harvested 7 h later. (C–F) Spleens from mice (six animals per group) infected IP with control or FliC^ind GFP–S. Typhimurium for 24 h and treated with doxycycline and fucoidan for 3.5 h were harvested and prepared for flow cytometry. Neutrophils (CD45⁺ CD11b⁺ Ly6G^+high; C–E) and neutrophils with intracellular macrophage markers (CD45⁺ CD11b⁺ Ly6G^+high CD68⁺ F4/80⁺; F) were identified by flow cytometry. All gating was performed using FlowJo. All data are representative of two. All error bars represent SE. P-values were determined by a two-tailed Student’s t test (G–I) or two-way ANOVA (D–E); *, P < 0.05.