Figure 3.
Figure 3. Necrosis and necroptosis result in similar cell corpses. (A–G) WT BMMs were treated with (A) 3 µg/ml FlaTox, (B) 50 ng/ml TNF, 10 µM ZVAD, and 5 µM BV6, (C) 0.05% Saponin, or (D) 1 µM staurosporine for 2 h. (A–D) WGA-labeled, GFP-expressing, or fixed and DAPI-stained cells were imaged by confocal microscopy at 0 and 2 h after treatment. Quantitation of cellular markers was done in ImageJ by measuring the total intensity of ∼50 individual live and dead BMMs. (E–G) BMMs were infected with SPI-1 repressed GFP-expressing S. Typhimurium for 2.5 h before indicated treatment. (E) The number of bacteria/cell or viable CFU were determined as in Fig. 2. (G) Cell lysis by LDH release. Data are representative of two (A–D) and three (E–G) individual experiments. Error bars represent SE. P-values were determined by a two-tailed Student’s t test.

Necrosis and necroptosis result in similar cell corpses. (A–G) WT BMMs were treated with (A) 3 µg/ml FlaTox, (B) 50 ng/ml TNF, 10 µM ZVAD, and 5 µM BV6, (C) 0.05% Saponin, or (D) 1 µM staurosporine for 2 h. (A–D) WGA-labeled, GFP-expressing, or fixed and DAPI-stained cells were imaged by confocal microscopy at 0 and 2 h after treatment. Quantitation of cellular markers was done in ImageJ by measuring the total intensity of ∼50 individual live and dead BMMs. (E–G) BMMs were infected with SPI-1 repressed GFP-expressing S. Typhimurium for 2.5 h before indicated treatment. (E) The number of bacteria/cell or viable CFU were determined as in Fig. 2. (G) Cell lysis by LDH release. Data are representative of two (A–D) and three (E–G) individual experiments. Error bars represent SE. P-values were determined by a two-tailed Student’s t test.

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