Immunotherapy induces differential gene expression and antigen-presenting machinery in CD11c⁺ microglia. (A) The heat map depicts differential gene expression (y axis) in microglia (CD45^lo 7-AAD⁻ Thy1.2⁻ Gr1⁻ CD11b⁺ CD39⁺ CX3CR1-GFP⁺ [carrier] or CD11c-YFP⁻ [immunotherapy]) flow cytometrically sorted from the brains of day 12 immunotherapy (IT) recipients versus untreated carrier controls (C; x axis; n = 3 mice per group). See corresponding Table S2. (B) IPA software was used to identify enriched functions and pathways from the microarray data. A complete list is provided in Table S2. The pie graph depicts the number of differentially regulated genes that intersect with two enriched IPA functions: proliferation of cells (green) and organismal death (red). The corresponding activation prediction based on Z-score is also provided. (C and D) IPA software was used to generate the interacting networks of genes associated with antigen presentation (C) and CD11c expression (D). Genes that were up-regulated in microglia from day 12 immunotherapy recipients are shown in shades of green, whereas down-regulated genes are shades of red. Data are derived from a single experiment.