Tailored chemokine expression and immune infiltration is observed in the brain after immunotherapy. (A and B) The absolute number of CD8⁺ T cells (CD45⁺ CD8⁺), P14 T cells (CD45⁺ CD8⁺ Thy1.1⁺), CD4⁺ T cells (CD45⁺ CD4⁺), and peripherally derived myeloid cells (CD45^hi Thy1.2⁻ CD11b⁺ Ly6C⁺ and CD45^hi Thy1.2⁻ CD11b⁺ Ly6G⁺) in the brain (A) and liver (B) was determined at day 9 after immunotherapy and compared with mice with LCMV meningitis (day 6) and naive controls (n = 4 mice per group). Asterisks indicate a statistically significant difference (*, P < 0.05) relative to the meningitis control group. (C) The denoted chemokines were quantified by Q-PCR using brain tissue from untreated carriers, day 9 immunotherapy recipients, or day 6 meningitis mice (n = 3 mice per group). Asterisks indicate a significant difference (*, P < 0.05) between the two groups. All bar graphs shown in this figure depict the mean ± SD, and data are representative of two (C) or three (A and B) independent experiments.