Lack of dural lymphatic vasculature inhibits CSF uptake into the dcLNs. (A) Schematic illustration of the experimental setup. (B) Representative fluorescent images of the dcLN in TG and WT mice 30 min after PEG-IRDye injection into the cisterna magna. AF, green channel autofluorescence. Bar, 1,000 µm. (C) Quantification of the dcLN fluorescence. n = 6 (TG) and 5 (WT). Data are representative of two independent experiments. Error bars indicate SD. Statistical analysis: two-tailed Student’s t test. *, P < 0.05.