Figure 2.
Figure 2. Treatment with IL-28A dramatically reduces proinflammatory IL-17–producing Th17 and γδ T cells in the joints. (a) Total numbers of Th17 and IL-17–producing γδ T cells in the hind paw arthritic joints of mice on the 10th day of treatment with IL-28A (0.4 mg/kg/day) or PBS control. The data are mean and SEM derived from eight mice treated with IL-28A and eight mice treated with PBS in a representative of three CIA experiments. Fold induction of (b) IL-17A, IL-1b, and IL-23a mRNA in dissociated cells from arthritic joints of mice treated with IL-28A or control. The data are mean and SEM derived from 19 mice treated with IL-28A and 19 mice treated with PBS from 3 independent CIA experiments. (c) Total numbers of CD4+ T cells and γδ T cells in the lymph nodes and blood of PBS and IL-28A–treated mice. The data are mean and SEM derived from 15 mice that developed arthritis, 8 treated with IL-28A, in a representative of three late CIA experiments. (d) Levels of IFN-γ, IL-1β, IL-6, and CXCL1 in the serum of mice on the 10th day of treatment with IL-28A or PBS control. The data are shown as mean with 95% confidence interval using 16 mice treated with IL-28A and 16 mice treated with PBS, from 3 independent late CIA experiments. (e) Proliferation of lymphocytes after 48-h stimulation with αCD3 or bovine CII. The data are mean and SEM derived from nine independent lymphocyte cultures from LNs of arthritic mice, four of which were treated with IL-28A. Statistical analysis was performed by one-tailed paired Student’s t test. (f) Levels of collagen-specific IgG1 and IgG2a in the serum. The data are mean and SEM using eight mice treated with IL-28A and eight mice treated with PBS, from a representative out of three independent late CIA experiments. (g) Total number of B cells in the lymph nodes. The data are mean and SEM derived from nine independent lymphocyte preparations from LNs of arthritic mice, four treated with IL-28A. Statistical analysis was performed by two-tailed Mann-Whitney U test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Treatment with IL-28A dramatically reduces proinflammatory IL-17–producing Th17 and γδ T cells in the joints. (a) Total numbers of Th17 and IL-17–producing γδ T cells in the hind paw arthritic joints of mice on the 10th day of treatment with IL-28A (0.4 mg/kg/day) or PBS control. The data are mean and SEM derived from eight mice treated with IL-28A and eight mice treated with PBS in a representative of three CIA experiments. Fold induction of (b) IL-17A, IL-1b, and IL-23a mRNA in dissociated cells from arthritic joints of mice treated with IL-28A or control. The data are mean and SEM derived from 19 mice treated with IL-28A and 19 mice treated with PBS from 3 independent CIA experiments. (c) Total numbers of CD4+ T cells and γδ T cells in the lymph nodes and blood of PBS and IL-28A–treated mice. The data are mean and SEM derived from 15 mice that developed arthritis, 8 treated with IL-28A, in a representative of three late CIA experiments. (d) Levels of IFN-γ, IL-1β, IL-6, and CXCL1 in the serum of mice on the 10th day of treatment with IL-28A or PBS control. The data are shown as mean with 95% confidence interval using 16 mice treated with IL-28A and 16 mice treated with PBS, from 3 independent late CIA experiments. (e) Proliferation of lymphocytes after 48-h stimulation with αCD3 or bovine CII. The data are mean and SEM derived from nine independent lymphocyte cultures from LNs of arthritic mice, four of which were treated with IL-28A. Statistical analysis was performed by one-tailed paired Student’s t test. (f) Levels of collagen-specific IgG1 and IgG2a in the serum. The data are mean and SEM using eight mice treated with IL-28A and eight mice treated with PBS, from a representative out of three independent late CIA experiments. (g) Total number of B cells in the lymph nodes. The data are mean and SEM derived from nine independent lymphocyte preparations from LNs of arthritic mice, four treated with IL-28A. Statistical analysis was performed by two-tailed Mann-Whitney U test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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