Release of hematopoietic stem and progenitor cells into blood after CD115 knockdown. (A) BrdU⁺ LSKs, HSCs, and GMPs (top). Quantification of BrdU⁺ cell % (middle). (bottom) Total BrdU⁺ LSKs, HSCs, and GMPs in the spleen after siRNA treatment (n = 3). (B) CFSE dilution of transferred LSKs (n = 3). (C) mRNA levels of HSC retention factors in (C) bone marrow and (D) spleen (n = 5–6). (E) LSKs in blood after siRNA treatment (n = 4–12). Data were pooled from at least two independent experiments. A and B depict one of two independent experiments. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.