Reduced HSC and progenitor levels in the spleen and bone marrow after CD115 knockdown. (A) Gating for LSKs, HSCs, and GMPs. FACS enumeration of HSCs, LSKs, and GMPs in the spleen (B) and bone marrow (C) after siRNA treatment (n = 3–19). Quantification of apoptotic bone marrow LSKs and HSCs (D) and splenic LSKs (E) after CD115 knockdown (n = 4–5). Data were pooled from 4 (B and C) experiments. (D and E) One of two independent experiments. Data are mean ± SEM. Statistical significance was determined by one-way ANOVA. **, P < 0.01; ***, P < 0.001.