Infiltration of BMDCs into the CNS in the cuprizone model does not impact demyelination or remyelination. (A) Staining of myelin with Black Gold II was performed on brain sections from GFP → WT and CCR2^−/− → WT chimeric mice. Representative micrograph images show Black Gold staining of untreated (left), GFP → WT (middle), and CCR2 → WT (right) chimeric mice after 6 wk of treatment with cuprizone or normal chow. (B and C) Demyelination (B) and remyelination (C) were measured in the corpus callosum of either GFP → WT or CCR2^−/− → WT after 6 wk of treatment with cuprizone (B) or 2 wk of normal chow after 6 wk of cuprizone (C). Differences between the GFP and CCR2^−/− groups were found not significant, with adjusted p-values over 0.8 in both cases in a one-way ANOVA followed by Turkey post-hoc test for multiple comparisons. One representative experiment out of two is shown. n = 3–10 mice/group. Bars, 100 µm.