Proinflammatory monocytes home to site of sterile injury and facilitate tissue repair. (a–c) Images from 8 to 48 h after focal necrotic injury of the liver demonstrating the response of Ccr2^RFP/+ (left) and Ccr2^RFP/RFP monocytes (right). (a) CCR2-RFP monocytes (red), (b) necrotic cells (green), (c) overlay. Bars, 200 µm. Data are representative of at least four independent experiments. (d and e) Quantification of RFP monocytes in Ccr2^RFP/+ and Ccr2^RFP/RFP mice (measured by percentage of area covered by RFP) either surrounding lesion (d) or within the lesion (e). Data are representative of at least two independent experiments, each with three mice per group. (f) Quantification of dead cells (measured as percentage of area covered by Sytox green) within the lesion. (g) Quantification of monocytes per field of view (FOV) in the liver from sham-operated Ccr2^RFP/+ and Ccr^RFP/RFP mice. (d–g) n = 6 mice per group; error bars are the SEM. (h) Monocytes were harvested from the bone marrow of Ccr2^RFP/+ and Ccr2^RFP/RFP mice, mixed 1:1 and i.v. transferred to a WT recipient 6 h after focal injury. 24 h after transfer, the ratio of Ccr2^RFP/+:Ccr2^RFP/RFP monocytes were measured in the blood and surrounding the liver injury. Data are representative of one experiment with three mice per group.