Neutrophils and monocytes traffic to site of hepatic sterile injury. (a, left) Mosaic of high-power images of CCR2⁺ cells in livers of Ccr2^RFP/+ mice visualized by SD-IVM. Autofluorescent hepatocytes (green); RFP⁺ cells (red). Bar, 100 µm. (right) Magnification of area outlined in white. Bar, 50 µm. (b and c) Time lapse (0 to 4 h) images from a Ccr2^RFP/+ (b) or a LysM-eGFP mouse (c) demonstrating the response of CCR2⁺ monocytes (red, b) or neutrophils (green, c) to focal hepatic injury (Sytox; green in b; propidium iodide; red in c). Bars, 200 µm. (d and e) Quantification of RFP⁺ monocytes (measured by % of area covered by RFP) either surrounding lesion (d) or within the lesion (e) following focal injury in Ccr2^RFP/+ mice. Mice were pretreated with anti-Ly6G to deplete neutrophils or with Rat IgG2a as control. Error bars are the SEM. (d and e) Data are representative of at least four independent experiments.