Decreased CHS response to DNFB by RvE1. (A) Flow cytometric analysis for dLN cell composition 5 d after 0.5% DNFB application in the presence or absence of RvE1 treatment. Bars indicate the numbers in each cell subset (n = 4). (B) [³H]thymidine incorporation by dLN cells after DNBS stimulation. Cell suspensions of dLNs from DNFB-sensitized mice were cultured for 3 d with or without 50 or 100 µg/ml DNBS. Cells were pulsed with 0.5 µCi [³H]thymidine for the last 24 h and subjected to liquid scintillation counting. (C) ELISA analysis for measuring IFN-γ in the culture medium 48 h after the culture. (D) Ear thickness change at 24 and 48 h after the challenge with 0.3% DNFB. WT and BLT1-deficient mice were treated with or without RvE1 (200 ng/mouse) 1 d and 30 min before sensitization (n = 4). Results are expressed as the mean ± SEM. All p-values were obtained by Student’s t test: *, P < 0.05. All data are representative of three independent experiments with reproducible results.