Bone mass analyses in EBI2- and CH25H-deficient mice. (A) Representative μCT images of femurs from 14-wk-old male WT (left), EBI2-deficient (middle), and CH25H-deficient mice (right). (B) Ratio of bone volume (BV) and trabecular volume (TV). (C) Number of trabecular bones (Tb.N.). (D) Spacing between trabecular bones (Tb.Sp., mm). (A–D) Data were generated by μCT of femurs, n = 10–12; four independent experiments. (E) Concentration of CTXs in serum of 14-wk-old male EBI2-deficient, CH25H-deficient, and control littermate mice, n = 4–6; three independent experiments. (F) Percentage of bone loss 4 wk after ovariectomy. Control (white), EBI2-deficient (green), and CH25H-deficient (blue) mice. (G) Ratio of bone volume and trabecular volume in 1-yr-old female WT and EBI2-deficient mice. (F–G) Data were generated by μCT of femurs, n = 7–12; three independent experiments. (B–G) Bars indicate mean, and circles depict individual mice analyzed. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by unpaired Student’s t test. See also Table 1 and Table S1.