Figure 4.
Figure 4. Assembly of the NLRP3/ASC inflammasome requires HOIL-1L. (a) Representative images of ASC foci formation (arrows) upon NLRP3 stimulation in WT or HOIL-1L−/− BMDMs with ASC staining in green, linear ubiquitin in red, and nuclei in blue. Bar, 20 µm. Images are from one of four independent experiments. (b) Quantification of ASC foci in 100 cells counted from images in a. (c) Z-stack of WT BMDMs in a. Bar, 10 µm. (d) Representative images of ASC foci formation (arrows) upon NLPR3 stimulation in HOIL-1L−/− and WT BMDMs infected with indicated lentiviruses and stained as described for a. Bar, 20 µm. Images are from one of two independent experiments. (e) Quantification of ASC foci from 30 cells combined from three experiments from images in d. (f) IP of ASC from untreated WT and HOIL-1L−/− BMDMs or after LPS priming and nigericin stimulation, data are representative of four independent experiments. Protein size markers are indicated in kD.

Assembly of the NLRP3/ASC inflammasome requires HOIL-1L. (a) Representative images of ASC foci formation (arrows) upon NLRP3 stimulation in WT or HOIL-1L−/− BMDMs with ASC staining in green, linear ubiquitin in red, and nuclei in blue. Bar, 20 µm. Images are from one of four independent experiments. (b) Quantification of ASC foci in 100 cells counted from images in a. (c) Z-stack of WT BMDMs in a. Bar, 10 µm. (d) Representative images of ASC foci formation (arrows) upon NLPR3 stimulation in HOIL-1L−/− and WT BMDMs infected with indicated lentiviruses and stained as described for a. Bar, 20 µm. Images are from one of two independent experiments. (e) Quantification of ASC foci from 30 cells combined from three experiments from images in d. (f) IP of ASC from untreated WT and HOIL-1L−/− BMDMs or after LPS priming and nigericin stimulation, data are representative of four independent experiments. Protein size markers are indicated in kD.

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