Global transcriptional profiles of WT and HOIL-1L^−/− BMDMs in response to LPS and TNF. (a) Heat map of genes with fivefold or greater changes in expression by whole genome microarray comparison were grouped by hierarchal clustering for WT and HOIL-1L^−/− BMDMs treated with 1 ng/ml TNF for 4 h, 10 ng/ml LPS for 4 h, or untreated for 4 h (biological n = 2, data for both individual replicates are presented as samples 1 and 2 for each condition). (b) Heat map of selected NF-κB genes compared by hierarchal clustering (biological n = 2, data for both individual replicates are presented as samples 1 and 2 for each condition). (c) Analysis of mRNA transcript abundance by qRT-PCR for selected genes as indicated in WT and HOIL-1L^−/− BMDMs stimulated as in a (biological n = 2 and analytical n = 2 for each biological replicate). All data are presented as the mean ± SEM by a Student’s t test.