Eosinophil accumulation in the lung in response to C. elegans dauers. (A) Tiled composite image of a DAPI (magenta) stained tissue section of the entire left lung lobe (left) 24 h after i.v. injection of 500 C. elegans dauers. Multiple inflammatory foci are visible (arrowheads). High-power inset (right) shows a larva surrounded by inflammatory cells, including eMBP⁺ eosinophils. The dauer exhibits nonspecific fluorescence. Bar, 50 µm. (B–D) Flow cytometry analysis of eosinophil accumulation in the lung at various time points after injection of C. elegans dauers (B and C) or H. bacteriophora larvae (D). (E) Flow cytometry analysis of alternatively activated macrophages (AAMs) in the lung 24 h after injection of C. elegans dauers or saline. (A–E) Results represent two (A–C) or are pooled from two (D and E) independent experiments. Horizontal bars indicate the mean.