Involvement of LTB4 in collective eosinophil migration. (A) Migration of eosinophils across Transwell membranes in response to CCL11 or leukotrienes. (B) LTB4 and LTE4 production in wild-type or Alox5−/− eosinophils treated with 1 µM ionomycin. Where indicated, cells were treated with 10 µM MK886. (C) LTB4 and LTE4 production in wild-type eosinophils incubated with C. elegans dauers. Where indicated, cells were treated with 10 µM MK886. (D) Accumulation of wild-type and Ltb4r1−/− eosinophils in response to C. elegans dauers (n ≥ 12 per experiment). (E) Eosinophil (Eos) and neutrophil (Neut) accumulation around C. elegans dauers (n ≥ 18). (F) Accumulation of wild-type and Alox5−/− neutrophils around IgG1- or F(ab′)2-coated agarose beads (n ≥ 8). (A–F) Results represent two (A–C, E, and F) or are pooled from four (D) independent experiments. Error bars indicate standard error of the mean. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.
