mRNA expression profiles of Tfh cells with different levels of S1pr2 expression. (A) mRNA expression profiles of Venus^hi Tfh, Venus^lo Tfh, PD-1^int Th, PD-1^lo Th, and naive CD4⁺ T cells are shown as heat map. See Fig. S3 A for the details of cell sorting. Each column represents a single experiment pooled from 3 to 8 (Venus^hi Tfh, Venus^lo Tfh, PD-1^int Th, PD-1^lo Th) or 1 to 2 (naive CD4⁺ T) mice. (B and C) The cytokine reporter (hCD2)–expressing cell percentages in Venus^lo or Venus^hi Tfh cells of S1pr2^V/+Il4-reporter mice (B) and S1pr2^V/+Il21-reporter mice (C). Draining LN cells were analyzed 9 or 11 d after immunization with CGG in CFA s.c. Data are pooled from two independent experiments with 8 LNs from 2 mice (B) or 7 LNs from 2 mice (C). Horizontal bars represent mean values. ***, P < 0.001; ****, P < 0.0001 (Student’s t test).