Figure 7.
Figure 7. IFN-γ acts as potent secretagogue of goblet cell mucus. (A) PAS staining on histological sections of anti-CD3–injected mice (same experiment as in Figure 6). Black arrows show massive extrusion of goblet cell mucus in wild-type but not in IFN-γ KO mice. Black arrowheads mark unaffected goblet cells on ileal villus (top row); note the IFN-γ–dependent villus damage. For the colon magnified regions (middle row) and overview images (bottom row) are shown. Phenotypes were fully penetrant in n = 7 animals per group and results were reproduced in 2 independent experiments. (B) Mouse colon organoids were treated with IFN-γ for 24 h (5 ng/ml) and stained with UEA-1 lectin (top) or PAS (bottom). Images are representative of two independent experiments. Arrows label secretion of goblet cell mucus. Bars, 100 µm.

IFN-γ acts as potent secretagogue of goblet cell mucus. (A) PAS staining on histological sections of anti-CD3–injected mice (same experiment as in Figure 6). Black arrows show massive extrusion of goblet cell mucus in wild-type but not in IFN-γ KO mice. Black arrowheads mark unaffected goblet cells on ileal villus (top row); note the IFN-γ–dependent villus damage. For the colon magnified regions (middle row) and overview images (bottom row) are shown. Phenotypes were fully penetrant in n = 7 animals per group and results were reproduced in 2 independent experiments. (B) Mouse colon organoids were treated with IFN-γ for 24 h (5 ng/ml) and stained with UEA-1 lectin (top) or PAS (bottom). Images are representative of two independent experiments. Arrows label secretion of goblet cell mucus. Bars, 100 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal