Figure 5.
Figure 5. Tracking CD21− FDC progenitors. (A) CD21Cre Ubow+/+ chimeras were injected with an emulsion of CFA/OVA in their ears and rear footpads to trigger FDC remodeling in their draining LNs. 3 wk later, inflamed draining LNs were sectioned and stained for CD21/35 and RANK-L expression. (B) CD21Cre Ubow+/− µMT mice were adoptively transferred with 6 × 107 WT polyclonal B cells to trigger FDC development. 1 wk later, peripheral LNs were sectioned and stained for CD21/35 and RANK-L expression. In A and B, data are representative of 3 different experiments (3 mice per experiment, 4 LNs analyzed per mouse). Insets display high-magnification views of MRC and transitional and mature FDCs. (C) Confocal pictures acquired in all the experiments described in A and B were used to determine the colors and absolute numbers of MRCs, transitional and mature FDCs, and other cells present in B cell follicles. Bars, 25 µm.

Tracking CD21− FDC progenitors. (A) CD21Cre Ubow+/+ chimeras were injected with an emulsion of CFA/OVA in their ears and rear footpads to trigger FDC remodeling in their draining LNs. 3 wk later, inflamed draining LNs were sectioned and stained for CD21/35 and RANK-L expression. (B) CD21Cre Ubow+/− µMT mice were adoptively transferred with 6 × 107 WT polyclonal B cells to trigger FDC development. 1 wk later, peripheral LNs were sectioned and stained for CD21/35 and RANK-L expression. In A and B, data are representative of 3 different experiments (3 mice per experiment, 4 LNs analyzed per mouse). Insets display high-magnification views of MRC and transitional and mature FDCs. (C) Confocal pictures acquired in all the experiments described in A and B were used to determine the colors and absolute numbers of MRCs, transitional and mature FDCs, and other cells present in B cell follicles. Bars, 25 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal