Lymphatic function is required to increase lung compliance in late gestation. (A) Individual pressure-volume (P-V) curves for 5 Ccbe1^+/+ and 4 Ccbe1^−/− lungs examined from 3 independent litters at E18.5 are shown. (B) P-V curves from the samples in A are represented as mean and SEM. (C) The calculated compliance of Ccbe1^−/− lungs compared with that of wild-type littermates at E18.5. Error bars represent mean and SEM from 5 Ccbe1^+/+ and 4 Ccbe1^−/− lungs examined from 3 independent litters (P = 0.003). (D) Individual pressure-volume (P-V) curves for 5 Vegfr3^+/+ and 6 Vegfr3^kd/kd lungs examined from 2 independent litters at E18.5 are shown. (E) P-V curves from the samples in D are represented as mean and SEM. (F) The calculated compliance of Vegfr3^kd/kd lungs compared with that of wild-type littermates at E18.5. Error bars represent mean and SEM from 5 Vegfr3^+/+ and 6 Vegfr3^kd/kd lungs examined from 2 independent litters (P > 0.05). Asterisk indicates P < 0.05 compared with control.