Laminin211 and Jab1 influence each other’s expression. (A) Western blot for Jab1 protein in sciatic nerve homogenate of WT (P30), dy2J (P30), dy3K (P20), and β1 integrin^−/− (P30) mice. Quantification is reported as the mean of three different mice per genotype (three independent experiments) and represented as ratio Jab1/β-tubulin, assigning WT as 1. (B) Western blot for laminin chain α2 in sciatic nerve homogenate of WT and Jab1^−/− mice. Quantification is reported as the mean of three different mice per genotype) and represented as ratio laminin chain α2/calnexin. (C) Immunofluorescence staining for laminin chain α2 in P20 sciatic nerves of WT and Jab1^−/− mice. Bar, 50 µm. (D) Western blot for Jab1 in sciatic nerve homogenate of P20 WT and Fig4^−/− (plt/plt) mice. Quantification is reported as the mean of three different mice for WT and five mice for Fig4^−/−. (E) Western blot for Jab1 in sciatic nerve homogenate of P30 WT and Nrg1 type III^+/− mice. Quantification is reported as the mean of two different mice per genotype. (D and E) Calnexin was used as a loading control. Unpaired Student’s t test in A and paired Student’s t test in B, D, and E: *, P ≤ 0.05; ***, P ≤ 0.001. Error bars indicate SEM.