Abnormal cytoskeletal organization in elongated cells. In-gel confocal imaging for F-actin (phalloidin, red), α-tubulin (green), and nuclei (DAPI, blue), in normal human T cells transfected with nonspecific (NS), DOCK8, or CDC42 siRNA, or treated with the PAK1/2 inhibitor IPA3. Representative of three experiments. Bars, 10 µm.