Production of IL17A and IL22 in TTP^−/− mice is IL23 dependent. (A and B) Total RNA from dLN or skin of TTP^−/−, IL23p19^−/− TTP^−/−, TNFR1/2^−/− TTP^−/−, and IL17A^−/− TTP^−/− mice was extracted and analyzed by real-time RT-PCR. Mean ± SEM from 6–10 mice is shown. (C and D) Intracellular IL-17A or IL22 production assessed by flow cytometry on dLN and skin cell suspensions of TTP^−/−, IL23p19^−/− TTP^−/−, TNFR1/2^−/− TTP^−/−, and IL17A^−/− TTP^−/− mice stimulated by PMA/ionomycin for 4 h. Mean ± SEM from 6–14 mice is shown. The horizontal bar represents the background staining obtained with isotype control antibodies (E) Flow cytometric analysis of cells in the spleen of TTP^+/+, TTP^−/−, IL23p19^−/− TTP^−/−, TNFR1/2^−/− TTP^−/−, and IL17A^−/− TTP^−/− mice. Mean ± SEM from 4–8 mice is shown. (F) ELISA analysis of the G-CSF present in sera of TTP^+/+, TTP^−/−, IL23p19^−/− TTP^−/−, TNFR1/2^−/− TTP^−/−, and IL17A^−/− TTP^−/− mice. Mean ± SEM from 12–20 mice is shown. Significant statistics are shown: *, P < 0.05; **, P < 0.01; ***, P < 0.001.