Enhanced IL17 and IL22 responses as consequence of the increased IL23 production. (A) Total RNA from dLN or skin of TTP^+/+ or TTP^−/− mice was extracted and analyzed by real-time RT-PCR. Mean ± SEM from 10–15 mice is shown. (B) Representative IL23p19-stained sections of the skin of TTP^+/+, TTP^−/−, or p19^−/− mice. Bar, 100 µm. (C) Total RNA from dLN or skin of TTP^+/+ or TTP^−/− mice was extracted and analyzed by real-time RT-PCR. Mean ± SEM from 4–10 mice is shown. (D and G) Intracellular IL17A, IL22, IFN-γ, or TNF production assessed by flow cytometry on dLN and spleen cell suspensions of TTP^+/+ and TTP^−/− mice stimulated by PMA/ionomycin for 4 h. Mean ± SEM from 6–10 mice is shown. (E) Naive CD4 T cells were stimulated with anti-CD3 and anti-CD28 and culture for 3 d under Th0, Th1, Th17, or Th22 conditions. Supernatants were collected analyzed for IL17A, IL22, and IFN-γ levels by ELISA. The results represent mean ± SEM of triplicates from one representative experiment of three. (F) Intracellular IL17A, IL22, IFN-γ, or TNF production assessed by flow cytometry on dLN and skin cell suspensions of TTP^+/+ and TTP^−/− mice stimulated by PMA/ionomycin for 4 h. Mean ± SEM from 6–10 mice is shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.