Expression of CCL1 in the mouse skin, tumor, and lymph node lymphatic vessels. (A–O) Double immunostaining for either LYVE-1 or podoplanin (lymphatic vessels, red) and mouse CCL1 (green). (A–C) Mouse skin overlying the tumor. (D–F) MDAcl.13 tumor xenograft. (G–L) Capsule and the lymph node cortex of the naive (G–I) and the tumor-draining (J–L) lymph node. (M–O) Lymph node medullary sinuses staining negative for CCL1. Images are representative at least n = 5 samples per group. ep, epidermis. Bars, 100 µm.