BATF KO gut T cells are deficient in expression of CCR9 and α4β7. (A) Expression of the indicated trafficking receptors by CD4⁺ FoxP3^+/− T cell subsets were analyzed by flow cytometry. (B) Graphs show absolute cell numbers. (C) Expression of CCR9 and α4β7 by CD8⁺ T cells. Graphs show frequencies of CCR9⁺ or α4β7⁺ CD8⁺ T cells in the indicated organs. Representative (A) and pooled (n = 8–11 for B; n = 5 for C) data obtained from at least five experiments using 6–8-wk-old mice are shown. All error bars are SEM obtained from pooled data. Significant differences between WT and BATF KO T cells are shown (*, P < 0.05).