Figure 1.
Figure 1. T cells form stable F-actin rings on bilayers containing pMHC and ICAM-1. (A) OT-1 CTLs were stimulated for 10 min on bilayers containing the indicated ligands, fixed, and stained with fluorescently labeled phalloidin. Representative TIRF images are shown. Images are representative of at least 100 cells imaged over at least two independent experiments. (B) OT-1 CTLs expressing RFP-labeled Lifeact together with GFP-labeled WAVE2 were analyzed by TIRF microscopy on bilayers containing pMHC and ICAM-1. A representative time-lapse montage (9-s intervals) is shown. Images are representative of at least 150 cells imaged over four independent experiments. Bars, 5 µm.

T cells form stable F-actin rings on bilayers containing pMHC and ICAM-1. (A) OT-1 CTLs were stimulated for 10 min on bilayers containing the indicated ligands, fixed, and stained with fluorescently labeled phalloidin. Representative TIRF images are shown. Images are representative of at least 100 cells imaged over at least two independent experiments. (B) OT-1 CTLs expressing RFP-labeled Lifeact together with GFP-labeled WAVE2 were analyzed by TIRF microscopy on bilayers containing pMHC and ICAM-1. A representative time-lapse montage (9-s intervals) is shown. Images are representative of at least 150 cells imaged over four independent experiments. Bars, 5 µm.

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