LAT vesicle fusion determines the number, density, and morphology of pLAT and pSLP76 nanoclusters at the immunological synapse. (A–G) Population analysis of pLAT and pSLP76 clusters in Jurkat cells processed as in Fig. 7. (A) Number of clusters per square micrometer is as follows: siCtr, n = 24; siSyt7, n = 21; siMAL, n = 14; siMAL TPS, n = 11; αCD45, n = 20; and αCD45 TPS, n = 22. (B) Number of clusters per square micrometer is as follows: siCtr, n = 18; siSyt7, n = 21; siMAL, n = 14; siMAL TPS, n = 11; αCD45, n = 19; and αCD45 TPS, n = 22. (C and D) Number of pLAT (C) and pSLP76 (D) detections per individual cluster for each cell analyzed for n ≥ 11 cells. (E) Mean pLAT cluster area per cell, n ≥ 11 cells. (F) Percentage of clusters whose circularity is equal to 1 per cell. Circularity value is given by the ratio between the large and the small feret diameters for all the clusters detected n ≥ 11 cells. (G) Mean area of pLAT clusters per cell according to circularity, measured as in F (n = 24). ***, P ≤ 0.0001; **, P ≤ 0.01; *, P ≤ 0.05; Mann-Whitney test. Each circle represents a cell. Representative of three experiments.